Journal: Journal of Cosmetic Dermatology

Article Title: Protective Effects of Exogenous Donkey Oil on Skin Healing Under Incisional Wound Damage

doi: 10.1111/jocd.70550

Figure Lengend Snippet: Effects of different concentrations of DO on the content of VEGF (A) and MMP‐9 (B) in the skin tissue of mice after incision injury. # p < 0.05, ### p < 0.001 vs. BC group; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. NC group.

Article Snippet: The ELISA kits IL‐1α and VEGF were purchased from ProteinTech (Wuhan, China, Cat No: KE10024 and KE10009); IL‐6 from Multi Sciences (Hangzhou, China, Cat No: EK206/3‐96); PGE2 from Lanpaibio (Shanghai, China, Cat No: LP‐M05161); MMP‐9 from CUSABIO (Wuhan, China, Cat No: CSB‐E08007m).

Techniques:

Journal: Scientific Reports

Article Title: Host-directed therapy in foals can enhance functional innate immunity and reduce severity of Rhodococcus equi pneumonia

doi: 10.1038/s41598-021-82049-y

Figure Lengend Snippet: Broncho-alveolar lavage (BAL) fluid differential cytology ( A – C ) and serum amyloid A (SAA; ( D )) of foals either before (Pre-nebulization) or after (Post-nebulization) serial nebulization with PUL-042 at various concentrations: 0 (n = 12), 2× (n = 12), 4× (n = 12), or 6× (n = 12). All foals were nebulized and no foals were infected in vivo with Rhodococcus equi . BALs and blood collection were performed pre- (day 2 after birth) and post-nebulization (day 22, 1 day after the last nebulization). Mean is represented by the symbol (circle for pre-nebulization; diamond for post-nebulization) and 95% confidence interval (whiskers). Percentage of macrophages ( A ) in BAL increased significantly (P < 0.0001) while percentage of neutrophils ( B ) decreased (P < 0.0001) after treatment (post) in all groups. The percentage of lymphocytes ( C ) did not vary significantly. Serum amyloid A ( D ) significantly decreased post-nebulization in all groups irrespective of treatment (P < 0.0001).

Article Snippet: BAL fluid , IL-1α , Kingfisher Biotech, Inc , St. Paul, MN , DIY0698E-003.

Techniques: Infection, In Vivo

Journal: Scientific Reports

Article Title: Host-directed therapy in foals can enhance functional innate immunity and reduce severity of Rhodococcus equi pneumonia

doi: 10.1038/s41598-021-82049-y

Figure Lengend Snippet: Concentration of pro- and anti-inflammatory cytokines in serum (left panel) or broncho-alveolar lavage (BAL; right panel) fluid of foals either before (Pre-nebulization) or after (Post-nebulization) serial nebulization with PUL-042 at various concentrations: 0 (n = 12), 2× (n = 12), 4× (n = 12), or 6× (n = 12). Cytokines were measured per ml of either serum or BAL fluid (TNF-α = pg/ml; IL-6 = pg/ml; IL-10 = ng/ml). All foals were nebulized and no foals were infected in vivo with Rhodococcus equi . BALs were performed pre (day 2 after birth) and post (day 22, 1 day after the last nebulization). In serum, TNF-⍺ ( A ) and IL-6 ( B ) significantly decreased with age, but only IL-6 had 1 group (PUL-042 2X) that was significantly different than PUL-042 0. Serum concentration of IL-10 ( C ) did not significantly differ among treatment groups or age. Tumor necrosis factor-alpha significantly increased in BAL fluid ( D ) for all groups, but effects were higher in BAL fluid of PUL-042 treated animals. The concentration of IL-6 ( E ) and IL-10 ( F ) in BAL fluid also did not significantly change with either age or treatment. Mean is represented by the symbol (circle for pre-nebulization; diamond for post-nebulization) and 95% confidence interval (whiskers).

Article Snippet: BAL fluid , IL-1α , Kingfisher Biotech, Inc , St. Paul, MN , DIY0698E-003.

Techniques: Concentration Assay, Infection, In Vivo

Journal: Scientific Reports

Article Title: Host-directed therapy in foals can enhance functional innate immunity and reduce severity of Rhodococcus equi pneumonia

doi: 10.1038/s41598-021-82049-y

Figure Lengend Snippet: ELISA commercial kits and manufacturers used in the study.

Article Snippet: BAL fluid , IL-1α , Kingfisher Biotech, Inc , St. Paul, MN , DIY0698E-003.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Turkish Journal of Chemistry

Article Title: Synthesis and characterization of new hexahydroquinoline derivatives and evaluation of their cytotoxicity, intracellular ROS production, and inhibitory effects on inflammatory mediators

doi: 10.55730/1300-0527.3686

Figure Lengend Snippet: Inflammation markers and and complement protein levels in the study groups. IL-1α: interleukin-1-alpha; IL-10: interleukin-10; TGF-β1: transforming growth factor-1-beta; TNF-α: tumor necrosis factor-alpha; C3: complement C3; C9: complement C9.

Article Snippet: TGF-β1, IL-1a, IL-10, TNF-α, C3, and C9 kits were purchased from BT Lab (Birmingham, UK).

Techniques: